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fas pcdna3 trail r2 ectopic gene expression gift  (Addgene inc)


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    Addgene inc fas pcdna3 trail r2 ectopic gene expression gift
    Fas Pcdna3 Trail R2 Ectopic Gene Expression Gift, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fas+pcdna3+trail+r2+ectopic+gene+expression+gift/pcDNA3-TRAIL-R2+(Plasmid+%2361383)/pmc12082145__BNEO_NEO-2024-000295-mmc1-99-150-159
    Average 93 stars, based on 2 article reviews
    fas pcdna3 trail r2 ectopic gene expression gift - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Western Blot:

    Article Title: Loss of p53 impairs death receptor expression and confers resistance to CD19 CAR T-cell therapy in BCP-ALL
    Article Snippet: L34974 1:4000 N/A N/A N/A N/A 4′,6-diamidino-2-phenylindole Sigma-Aldrich D9542 0,4 μg/mL N/A N/A N/A N/A 7-aminoactinomycin D BD Biosciences 559925 1:100 N/A N/A N/A N/A Immunoblotting Target Host Supplier Cat. number Solvent Dilution Incubation p53 Mouse SCBT sc-126 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT p21 Rabbit Cell Signaling Technology 2947 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT Vinculin Mouse Sigma-Aldrich V9131 1% milk in TBS/0.1%Tween-20 1:5000 1 hour, RT Plasmids Plasmid Application Source Relevant content pLNT-Sffv GFP-expressing cells Gift from Vaskar Saha GFP pLNT-Sffv mCherry-expressing cells Gift from Vaskar Saha mCherry pCCL-cPPT-hPGKFMC63CAR1941BBLCAR-bPRE4-SIN CD19-targeting CAR Generated in-house CD19-targeting CAR pMDL-g/pRRE Lentiviral transduction Gift from Didier Trono, Addgene #12251 Lentiviral packaging proteins pMD2-VSVg Lentiviral transduction Gift from Didier Trono, Addgene #12259 Lentiviral packaging proteins pRSV-Rev Lentiviral transduction Gift from Didier Trono, Addgene #12253 Lentiviral packaging proteins FAS-pLX307 Ectopic gene expression Gift from William Hahn and Sefi Rosenbluh, Addgene #98334 Fas pcDNA3-TRAIL-R2 Ectopic gene expression Gift from Francis Chan, Addgene #61383 DR5 construct DR5-pLX307 Ectopic gene expression DR5 was extracted from pcDNA3-TRAILR2 source plasmid and cloned into a pLX307 backbone (from FAS-pLX307) using PCR and EcoRV and NheI (Promega; R6351 and R6501, respectively) restriction digestion.

    Solvent:

    Article Title: Loss of p53 impairs death receptor expression and confers resistance to CD19 CAR T-cell therapy in BCP-ALL
    Article Snippet: L34974 1:4000 N/A N/A N/A N/A 4′,6-diamidino-2-phenylindole Sigma-Aldrich D9542 0,4 μg/mL N/A N/A N/A N/A 7-aminoactinomycin D BD Biosciences 559925 1:100 N/A N/A N/A N/A Immunoblotting Target Host Supplier Cat. number Solvent Dilution Incubation p53 Mouse SCBT sc-126 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT p21 Rabbit Cell Signaling Technology 2947 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT Vinculin Mouse Sigma-Aldrich V9131 1% milk in TBS/0.1%Tween-20 1:5000 1 hour, RT Plasmids Plasmid Application Source Relevant content pLNT-Sffv GFP-expressing cells Gift from Vaskar Saha GFP pLNT-Sffv mCherry-expressing cells Gift from Vaskar Saha mCherry pCCL-cPPT-hPGKFMC63CAR1941BBLCAR-bPRE4-SIN CD19-targeting CAR Generated in-house CD19-targeting CAR pMDL-g/pRRE Lentiviral transduction Gift from Didier Trono, Addgene #12251 Lentiviral packaging proteins pMD2-VSVg Lentiviral transduction Gift from Didier Trono, Addgene #12259 Lentiviral packaging proteins pRSV-Rev Lentiviral transduction Gift from Didier Trono, Addgene #12253 Lentiviral packaging proteins FAS-pLX307 Ectopic gene expression Gift from William Hahn and Sefi Rosenbluh, Addgene #98334 Fas pcDNA3-TRAIL-R2 Ectopic gene expression Gift from Francis Chan, Addgene #61383 DR5 construct DR5-pLX307 Ectopic gene expression DR5 was extracted from pcDNA3-TRAILR2 source plasmid and cloned into a pLX307 backbone (from FAS-pLX307) using PCR and EcoRV and NheI (Promega; R6351 and R6501, respectively) restriction digestion.

    Incubation:

    Article Title: Loss of p53 impairs death receptor expression and confers resistance to CD19 CAR T-cell therapy in BCP-ALL
    Article Snippet: L34974 1:4000 N/A N/A N/A N/A 4′,6-diamidino-2-phenylindole Sigma-Aldrich D9542 0,4 μg/mL N/A N/A N/A N/A 7-aminoactinomycin D BD Biosciences 559925 1:100 N/A N/A N/A N/A Immunoblotting Target Host Supplier Cat. number Solvent Dilution Incubation p53 Mouse SCBT sc-126 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT p21 Rabbit Cell Signaling Technology 2947 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT Vinculin Mouse Sigma-Aldrich V9131 1% milk in TBS/0.1%Tween-20 1:5000 1 hour, RT Plasmids Plasmid Application Source Relevant content pLNT-Sffv GFP-expressing cells Gift from Vaskar Saha GFP pLNT-Sffv mCherry-expressing cells Gift from Vaskar Saha mCherry pCCL-cPPT-hPGKFMC63CAR1941BBLCAR-bPRE4-SIN CD19-targeting CAR Generated in-house CD19-targeting CAR pMDL-g/pRRE Lentiviral transduction Gift from Didier Trono, Addgene #12251 Lentiviral packaging proteins pMD2-VSVg Lentiviral transduction Gift from Didier Trono, Addgene #12259 Lentiviral packaging proteins pRSV-Rev Lentiviral transduction Gift from Didier Trono, Addgene #12253 Lentiviral packaging proteins FAS-pLX307 Ectopic gene expression Gift from William Hahn and Sefi Rosenbluh, Addgene #98334 Fas pcDNA3-TRAIL-R2 Ectopic gene expression Gift from Francis Chan, Addgene #61383 DR5 construct DR5-pLX307 Ectopic gene expression DR5 was extracted from pcDNA3-TRAILR2 source plasmid and cloned into a pLX307 backbone (from FAS-pLX307) using PCR and EcoRV and NheI (Promega; R6351 and R6501, respectively) restriction digestion.

    Plasmid Preparation:

    Article Title: Loss of p53 impairs death receptor expression and confers resistance to CD19 CAR T-cell therapy in BCP-ALL
    Article Snippet: L34974 1:4000 N/A N/A N/A N/A 4′,6-diamidino-2-phenylindole Sigma-Aldrich D9542 0,4 μg/mL N/A N/A N/A N/A 7-aminoactinomycin D BD Biosciences 559925 1:100 N/A N/A N/A N/A Immunoblotting Target Host Supplier Cat. number Solvent Dilution Incubation p53 Mouse SCBT sc-126 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT p21 Rabbit Cell Signaling Technology 2947 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT Vinculin Mouse Sigma-Aldrich V9131 1% milk in TBS/0.1%Tween-20 1:5000 1 hour, RT Plasmids Plasmid Application Source Relevant content pLNT-Sffv GFP-expressing cells Gift from Vaskar Saha GFP pLNT-Sffv mCherry-expressing cells Gift from Vaskar Saha mCherry pCCL-cPPT-hPGKFMC63CAR1941BBLCAR-bPRE4-SIN CD19-targeting CAR Generated in-house CD19-targeting CAR pMDL-g/pRRE Lentiviral transduction Gift from Didier Trono, Addgene #12251 Lentiviral packaging proteins pMD2-VSVg Lentiviral transduction Gift from Didier Trono, Addgene #12259 Lentiviral packaging proteins pRSV-Rev Lentiviral transduction Gift from Didier Trono, Addgene #12253 Lentiviral packaging proteins FAS-pLX307 Ectopic gene expression Gift from William Hahn and Sefi Rosenbluh, Addgene #98334 Fas pcDNA3-TRAIL-R2 Ectopic gene expression Gift from Francis Chan, Addgene #61383 DR5 construct DR5-pLX307 Ectopic gene expression DR5 was extracted from pcDNA3-TRAILR2 source plasmid and cloned into a pLX307 backbone (from FAS-pLX307) using PCR and EcoRV and NheI (Promega; R6351 and R6501, respectively) restriction digestion.

    Generated:

    Article Title: Loss of p53 impairs death receptor expression and confers resistance to CD19 CAR T-cell therapy in BCP-ALL
    Article Snippet: L34974 1:4000 N/A N/A N/A N/A 4′,6-diamidino-2-phenylindole Sigma-Aldrich D9542 0,4 μg/mL N/A N/A N/A N/A 7-aminoactinomycin D BD Biosciences 559925 1:100 N/A N/A N/A N/A Immunoblotting Target Host Supplier Cat. number Solvent Dilution Incubation p53 Mouse SCBT sc-126 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT p21 Rabbit Cell Signaling Technology 2947 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT Vinculin Mouse Sigma-Aldrich V9131 1% milk in TBS/0.1%Tween-20 1:5000 1 hour, RT Plasmids Plasmid Application Source Relevant content pLNT-Sffv GFP-expressing cells Gift from Vaskar Saha GFP pLNT-Sffv mCherry-expressing cells Gift from Vaskar Saha mCherry pCCL-cPPT-hPGKFMC63CAR1941BBLCAR-bPRE4-SIN CD19-targeting CAR Generated in-house CD19-targeting CAR pMDL-g/pRRE Lentiviral transduction Gift from Didier Trono, Addgene #12251 Lentiviral packaging proteins pMD2-VSVg Lentiviral transduction Gift from Didier Trono, Addgene #12259 Lentiviral packaging proteins pRSV-Rev Lentiviral transduction Gift from Didier Trono, Addgene #12253 Lentiviral packaging proteins FAS-pLX307 Ectopic gene expression Gift from William Hahn and Sefi Rosenbluh, Addgene #98334 Fas pcDNA3-TRAIL-R2 Ectopic gene expression Gift from Francis Chan, Addgene #61383 DR5 construct DR5-pLX307 Ectopic gene expression DR5 was extracted from pcDNA3-TRAILR2 source plasmid and cloned into a pLX307 backbone (from FAS-pLX307) using PCR and EcoRV and NheI (Promega; R6351 and R6501, respectively) restriction digestion.

    Transduction:

    Article Title: Loss of p53 impairs death receptor expression and confers resistance to CD19 CAR T-cell therapy in BCP-ALL
    Article Snippet: L34974 1:4000 N/A N/A N/A N/A 4′,6-diamidino-2-phenylindole Sigma-Aldrich D9542 0,4 μg/mL N/A N/A N/A N/A 7-aminoactinomycin D BD Biosciences 559925 1:100 N/A N/A N/A N/A Immunoblotting Target Host Supplier Cat. number Solvent Dilution Incubation p53 Mouse SCBT sc-126 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT p21 Rabbit Cell Signaling Technology 2947 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT Vinculin Mouse Sigma-Aldrich V9131 1% milk in TBS/0.1%Tween-20 1:5000 1 hour, RT Plasmids Plasmid Application Source Relevant content pLNT-Sffv GFP-expressing cells Gift from Vaskar Saha GFP pLNT-Sffv mCherry-expressing cells Gift from Vaskar Saha mCherry pCCL-cPPT-hPGKFMC63CAR1941BBLCAR-bPRE4-SIN CD19-targeting CAR Generated in-house CD19-targeting CAR pMDL-g/pRRE Lentiviral transduction Gift from Didier Trono, Addgene #12251 Lentiviral packaging proteins pMD2-VSVg Lentiviral transduction Gift from Didier Trono, Addgene #12259 Lentiviral packaging proteins pRSV-Rev Lentiviral transduction Gift from Didier Trono, Addgene #12253 Lentiviral packaging proteins FAS-pLX307 Ectopic gene expression Gift from William Hahn and Sefi Rosenbluh, Addgene #98334 Fas pcDNA3-TRAIL-R2 Ectopic gene expression Gift from Francis Chan, Addgene #61383 DR5 construct DR5-pLX307 Ectopic gene expression DR5 was extracted from pcDNA3-TRAILR2 source plasmid and cloned into a pLX307 backbone (from FAS-pLX307) using PCR and EcoRV and NheI (Promega; R6351 and R6501, respectively) restriction digestion.

    Gene Expression:

    Article Title: Loss of p53 impairs death receptor expression and confers resistance to CD19 CAR T-cell therapy in BCP-ALL
    Article Snippet: L34974 1:4000 N/A N/A N/A N/A 4′,6-diamidino-2-phenylindole Sigma-Aldrich D9542 0,4 μg/mL N/A N/A N/A N/A 7-aminoactinomycin D BD Biosciences 559925 1:100 N/A N/A N/A N/A Immunoblotting Target Host Supplier Cat. number Solvent Dilution Incubation p53 Mouse SCBT sc-126 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT p21 Rabbit Cell Signaling Technology 2947 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT Vinculin Mouse Sigma-Aldrich V9131 1% milk in TBS/0.1%Tween-20 1:5000 1 hour, RT Plasmids Plasmid Application Source Relevant content pLNT-Sffv GFP-expressing cells Gift from Vaskar Saha GFP pLNT-Sffv mCherry-expressing cells Gift from Vaskar Saha mCherry pCCL-cPPT-hPGKFMC63CAR1941BBLCAR-bPRE4-SIN CD19-targeting CAR Generated in-house CD19-targeting CAR pMDL-g/pRRE Lentiviral transduction Gift from Didier Trono, Addgene #12251 Lentiviral packaging proteins pMD2-VSVg Lentiviral transduction Gift from Didier Trono, Addgene #12259 Lentiviral packaging proteins pRSV-Rev Lentiviral transduction Gift from Didier Trono, Addgene #12253 Lentiviral packaging proteins FAS-pLX307 Ectopic gene expression Gift from William Hahn and Sefi Rosenbluh, Addgene #98334 Fas pcDNA3-TRAIL-R2 Ectopic gene expression Gift from Francis Chan, Addgene #61383 DR5 construct DR5-pLX307 Ectopic gene expression DR5 was extracted from pcDNA3-TRAILR2 source plasmid and cloned into a pLX307 backbone (from FAS-pLX307) using PCR and EcoRV and NheI (Promega; R6351 and R6501, respectively) restriction digestion.

    Construct:

    Article Title: Loss of p53 impairs death receptor expression and confers resistance to CD19 CAR T-cell therapy in BCP-ALL
    Article Snippet: L34974 1:4000 N/A N/A N/A N/A 4′,6-diamidino-2-phenylindole Sigma-Aldrich D9542 0,4 μg/mL N/A N/A N/A N/A 7-aminoactinomycin D BD Biosciences 559925 1:100 N/A N/A N/A N/A Immunoblotting Target Host Supplier Cat. number Solvent Dilution Incubation p53 Mouse SCBT sc-126 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT p21 Rabbit Cell Signaling Technology 2947 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT Vinculin Mouse Sigma-Aldrich V9131 1% milk in TBS/0.1%Tween-20 1:5000 1 hour, RT Plasmids Plasmid Application Source Relevant content pLNT-Sffv GFP-expressing cells Gift from Vaskar Saha GFP pLNT-Sffv mCherry-expressing cells Gift from Vaskar Saha mCherry pCCL-cPPT-hPGKFMC63CAR1941BBLCAR-bPRE4-SIN CD19-targeting CAR Generated in-house CD19-targeting CAR pMDL-g/pRRE Lentiviral transduction Gift from Didier Trono, Addgene #12251 Lentiviral packaging proteins pMD2-VSVg Lentiviral transduction Gift from Didier Trono, Addgene #12259 Lentiviral packaging proteins pRSV-Rev Lentiviral transduction Gift from Didier Trono, Addgene #12253 Lentiviral packaging proteins FAS-pLX307 Ectopic gene expression Gift from William Hahn and Sefi Rosenbluh, Addgene #98334 Fas pcDNA3-TRAIL-R2 Ectopic gene expression Gift from Francis Chan, Addgene #61383 DR5 construct DR5-pLX307 Ectopic gene expression DR5 was extracted from pcDNA3-TRAILR2 source plasmid and cloned into a pLX307 backbone (from FAS-pLX307) using PCR and EcoRV and NheI (Promega; R6351 and R6501, respectively) restriction digestion.

    Clone Assay:

    Article Title: Loss of p53 impairs death receptor expression and confers resistance to CD19 CAR T-cell therapy in BCP-ALL
    Article Snippet: L34974 1:4000 N/A N/A N/A N/A 4′,6-diamidino-2-phenylindole Sigma-Aldrich D9542 0,4 μg/mL N/A N/A N/A N/A 7-aminoactinomycin D BD Biosciences 559925 1:100 N/A N/A N/A N/A Immunoblotting Target Host Supplier Cat. number Solvent Dilution Incubation p53 Mouse SCBT sc-126 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT p21 Rabbit Cell Signaling Technology 2947 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT Vinculin Mouse Sigma-Aldrich V9131 1% milk in TBS/0.1%Tween-20 1:5000 1 hour, RT Plasmids Plasmid Application Source Relevant content pLNT-Sffv GFP-expressing cells Gift from Vaskar Saha GFP pLNT-Sffv mCherry-expressing cells Gift from Vaskar Saha mCherry pCCL-cPPT-hPGKFMC63CAR1941BBLCAR-bPRE4-SIN CD19-targeting CAR Generated in-house CD19-targeting CAR pMDL-g/pRRE Lentiviral transduction Gift from Didier Trono, Addgene #12251 Lentiviral packaging proteins pMD2-VSVg Lentiviral transduction Gift from Didier Trono, Addgene #12259 Lentiviral packaging proteins pRSV-Rev Lentiviral transduction Gift from Didier Trono, Addgene #12253 Lentiviral packaging proteins FAS-pLX307 Ectopic gene expression Gift from William Hahn and Sefi Rosenbluh, Addgene #98334 Fas pcDNA3-TRAIL-R2 Ectopic gene expression Gift from Francis Chan, Addgene #61383 DR5 construct DR5-pLX307 Ectopic gene expression DR5 was extracted from pcDNA3-TRAILR2 source plasmid and cloned into a pLX307 backbone (from FAS-pLX307) using PCR and EcoRV and NheI (Promega; R6351 and R6501, respectively) restriction digestion.

    Polymerase Chain Reaction:

    Article Title: Loss of p53 impairs death receptor expression and confers resistance to CD19 CAR T-cell therapy in BCP-ALL
    Article Snippet: L34974 1:4000 N/A N/A N/A N/A 4′,6-diamidino-2-phenylindole Sigma-Aldrich D9542 0,4 μg/mL N/A N/A N/A N/A 7-aminoactinomycin D BD Biosciences 559925 1:100 N/A N/A N/A N/A Immunoblotting Target Host Supplier Cat. number Solvent Dilution Incubation p53 Mouse SCBT sc-126 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT p21 Rabbit Cell Signaling Technology 2947 1% milk in TBS/0.1%Tween-20 1:1000 1 hour, RT Vinculin Mouse Sigma-Aldrich V9131 1% milk in TBS/0.1%Tween-20 1:5000 1 hour, RT Plasmids Plasmid Application Source Relevant content pLNT-Sffv GFP-expressing cells Gift from Vaskar Saha GFP pLNT-Sffv mCherry-expressing cells Gift from Vaskar Saha mCherry pCCL-cPPT-hPGKFMC63CAR1941BBLCAR-bPRE4-SIN CD19-targeting CAR Generated in-house CD19-targeting CAR pMDL-g/pRRE Lentiviral transduction Gift from Didier Trono, Addgene #12251 Lentiviral packaging proteins pMD2-VSVg Lentiviral transduction Gift from Didier Trono, Addgene #12259 Lentiviral packaging proteins pRSV-Rev Lentiviral transduction Gift from Didier Trono, Addgene #12253 Lentiviral packaging proteins FAS-pLX307 Ectopic gene expression Gift from William Hahn and Sefi Rosenbluh, Addgene #98334 Fas pcDNA3-TRAIL-R2 Ectopic gene expression Gift from Francis Chan, Addgene #61383 DR5 construct DR5-pLX307 Ectopic gene expression DR5 was extracted from pcDNA3-TRAILR2 source plasmid and cloned into a pLX307 backbone (from FAS-pLX307) using PCR and EcoRV and NheI (Promega; R6351 and R6501, respectively) restriction digestion.



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    fas pcdna3 trail r2 ectopic gene expression gift  (Addgene inc)
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    Addgene inc fas pcdna3 trail r2 ectopic gene expression gift
    Fas Pcdna3 Trail R2 Ectopic Gene Expression Gift, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fas+pcdna3+trail+r2+ectopic+gene+expression+gift/pcDNA3-TRAIL-R2+(Plasmid+%2361383)/pmc12082145__BNEO_NEO-2024-000295-mmc1-99-150-159
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    fas pcdna3 trail r2 ectopic gene expression gift - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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